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Research on associations between variants and haplotypes of Aquaporin 9 (AQP9) gene with growth traits in three cattle breeds.

Authors
  • Zhang, Jingmin1, 2
  • Tan, Jiaoyan1
  • Zhang, Chunlei1
  • Wang, Yanhong1
  • Chen, Xi1
  • Lei, Chuzhao3
  • Chen, Hong1
  • Fang, Xingtang1
  • 1 Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, P. R. China. , (China)
  • 2 Shanghai Vocational College of Agriculture and Forestry, Shanghai, P. R. China. , (China)
  • 3 Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, P. R. China. , (China)
Type
Published Article
Journal
Animal biotechnology
Publication Date
Apr 01, 2021
Volume
32
Issue
2
Pages
185–193
Identifiers
DOI: 10.1080/10495398.2019.1675681
PMID: 31680615
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Aquaporin 9 plays critical roles in aspects of energy homeostasis, metabolism, gluconeogenesis, fat synthesis and even the individual growth and development. So the Aquaporin 9 (AQP9) gene is a potential candidate gene for bovine growth traits. In this study, we detected the polymorphism of the bovine AQP9 gene including all exons by PCR-SSCP and DNA sequencing methods with six pairs of PCR primers in 555 individuals from three cattle breeds. Three novel SNPs (NC_007308:g.47575 C > T, 47615 C > T, 47690A > G) were detected using P6 primer. The linkage disequilibrium analysis indicated that the three SNPs were completely linked (r2 = 1), which constructed three genotypes (AA, AB, BB). The genotype AB was dominant in all three breeds. The frequencies of haplotype A and haplotype B were almost equivalent between each other. The individuals with genotype AB were significantly higher than those individuals with genotype BB in body weight (p < 0.01), chest circumference (p < 0.05) and rump length (p < 0.05). Moreover, individuals with genotype AA were significantly higher than those of individuals with genotype BB in body height (p < 0.01). These results suggested that the novel SNPs could be a perfect molecular marker for marker-assisted selection (MAS) breeding.

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