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Repeated Oral Exposure to Nε-Carboxymethyllysine, a Maillard Reaction Product, Alleviates Gut Microbiota Dysbiosis in Colitic Mice

Authors
  • ALJahdali, Nesreen1
  • Gadonna-Widehem, Pascale2
  • Delayre-Orthez, Carine2
  • Marier, David2
  • Garnier, Benjamin2
  • Carbonero, Franck1, 3
  • Anton, Pauline M.2
  • 1 University of Arkansas, Cell and Molecular Biology Program, 2650 Young Avenue, Fayetteville, AR, 72704, USA , Fayetteville (United States)
  • 2 Institut Polytechnique UniLaSalle, Expression des Gènes et Régulation Epigénétique par l’Aliment UP 2012.10.101., 19 rue Pierre Waguet, Beauvais, 60000, France , Beauvais (France)
  • 3 University of Arkansas, Department of Food Science and Center for Human Nutrition, 2650 Young Avenue, Fayetteville, AR, 72704, USA , Fayetteville (United States)
Type
Published Article
Journal
Digestive Diseases and Sciences
Publisher
Springer-Verlag
Publication Date
Sep 30, 2017
Volume
62
Issue
12
Pages
3370–3384
Identifiers
DOI: 10.1007/s10620-017-4767-8
Source
Springer Nature
Keywords
License
Yellow

Abstract

BackgroundDiet is suggested to participate in the etiology of inflammatory bowel diseases (IBD). Repeated exposure to Maillard reaction products (MRPs), molecules resulting from reduction reactions between amino acids and sugars during food heating, has been reported to be either potentially detrimental or beneficial to health.AimsThe aim of this study is to determine the effect of repeated oral ingestion of Nε-carboxymethyllysine (CML), an advanced MRP, on the onset of two models of experimental IBD and on the gut microbiota composition of mice.MethodsMice received either saline (control) or Nε-carboxymethyllysine daily for 21 days. For the last week of treatment, each group was split into subgroups, receiving dextran sulfate sodium salt (DSS) or trinitrobenzenesulfonic acid (TNBS) to induce colitis. Intensity of inflammation was quantified, and cecal microbiota characterized by bacterial 16S ribosomal RNA (rRNA) amplicon sequencing.ResultsDaily oral administration of Nε-carboxymethyllysine did not induce intestinal inflammation and had limited impact on gut microbiota composition (Bacteroidaceae increase, Lachnospiraceae decrease). DSS and TNBS administration resulted in expected moderate experimental colitis with a shift of Bacteroidetes/Firmicutes ratio and a significant Proteobacteria increase but with distinct profiles: different Proteobacteria taxa for DSS, but mainly Enterobacteriaceae for TNBS. While Nε-carboxymethyllysine exposure failed to prevent the inflammatory response, it allowed maintenance of healthy gut microbiota profiles in mice treated with DSS (but not TNBS).ConclusionsRepeated oral exposure to CML limits dysbiosis in experimental colitis. IBD patients may modulate their microbiota profile by regulating the level and type of dietary MRP consumption.

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