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Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

Authors
  • Milessi, Thais S.1, 2
  • Perez, Caroline L.3
  • Zangirolami, Teresa C.1, 3
  • Corradini, Felipe A. S.3
  • Sandri, Juliana P.3
  • Foulquié-Moreno, Maria R.4, 5
  • Giordano, Roberto C.1, 3
  • Thevelein, Johan M.4, 5
  • Giordano, Raquel L. C.1, 3
  • 1 Federal University of São Carlos, Rodovia Washington Luís, km 235, São Carlos, SP, 13565-905, Brazil , São Carlos (Brazil)
  • 2 Federal University of Itajubá, Av. Benedito Pereira dos Santos, 1303, Itajubá, MG, 37500-903, Brazil , Itajubá (Brazil)
  • 3 Federal University of São Carlos (PPGEQ-UFSCar), Rodovia Washington Luís, km 235, São Carlos, SP, 13565-905, Brazil , São Carlos (Brazil)
  • 4 Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, Leuven-Heverlee, Flanders, 3001, Belgium , Leuven-Heverlee (Belgium)
  • 5 VIB, Kasteelpark Arenberg 31, Leuven-Heverlee, Flanders, 3001, Belgium , Leuven-Heverlee (Belgium)
Type
Published Article
Journal
Biotechnology for Biofuels
Publisher
Springer (Biomed Central Ltd.)
Publication Date
May 11, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13068-020-01722-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundThe search for sustainable energy sources has become a worldwide issue, making the development of efficient biofuel production processes a priority. Immobilization of second-generation (2G) xylose-fermenting Saccharomyces cerevisiae strains is a promising approach to achieve economic viability of 2G bioethanol production from undetoxified hydrolysates through operation at high cell load and mitigation of inhibitor toxicity. In addition, the use of a fixed-bed reactor can contribute to establish an efficient process because of its distinct advantages, such as high conversion rate per weight of biocatalyst and reuse of biocatalyst.ResultsThis work assessed the influence of alginate entrapment on the tolerance of recombinant S. cerevisiae to acetic acid. Encapsulated GSE16-T18SI.1 (T18) yeast showed an outstanding performance in repeated batch fermentations with cell recycling in YPX medium supplemented with 8 g/L acetic acid (pH 5.2), achieving 10 cycles without significant loss of productivity. In the fixed-bed bioreactor, a high xylose fermentation rate with ethanol yield and productivity values of 0.38 gethanol/gsugars and 5.7 g/L/h, respectively were achieved in fermentations using undetoxified sugarcane bagasse hemicellulose hydrolysate, with and without medium recirculation.ConclusionsThe performance of recombinant strains developed for 2G ethanol production can be boosted strongly by cell immobilization in alginate gels. Yeast encapsulation allows conducting fermentations in repeated batch mode in fixed-bed bioreactors with high xylose assimilation rate and high ethanol productivity using undetoxified hemicellulose hydrolysate.

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