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Remarkably Complex Microbial Community Composition in Bromeliad Tank Waters Revealed by eDNA Metabarcoding.

Authors
  • Simão, Taiz L L1
  • Utz, Laura R P2
  • Dias, Raquel3
  • Giongo, Adriana4
  • Triplett, Eric W5
  • Eizirik, Eduardo1
  • 1 Laboratório de Biologia Genômica e Molecular, Escola de Ciências da Saúde e da Vida, PUCRS, Av. Ipiranga, 6681, prédio 12., Porto Alegre, Rio Grande do Sul, 90619-900, Brazil. , (Brazil)
  • 2 Laboratório de Ecologia Aquática, Escola de Ciências da Saúde e da Vida, PUCRS, Av. Ipiranga, 6681, prédio 12., Porto Alegre, Rio Grande do Sul, 90619-900, Brazil. , (Brazil)
  • 3 Department of Integrative Structural and Computational Biology, Scripps Research Translational Institute, 3344 North Torrey Pines Court, Suite 300, La Jolla, California, 92037, USA.
  • 4 Instituto do Petróleo e Recursos Naturais, PUCRS, Av. Ipiranga 6681, prédio 96J, Porto Alegre, Rio Grande do Sul, 90619-900, Brazil. , (Brazil)
  • 5 Department of Microbiology and Cell Science, University of Florida, 1052 Museum Road, P.O. Box 110700, Gainesville, Florida, 32608, USA.
Type
Published Article
Journal
The Journal of eukaryotic microbiology
Publication Date
Sep 01, 2020
Volume
67
Issue
5
Pages
593–607
Identifiers
DOI: 10.1111/jeu.12814
PMID: 32562451
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To investigate patterns of biotic community composition at different spatial scales and biological contexts, we used environmental DNA metabarcoding to characterize eukaryotic and prokaryotic assemblages present in the phytotelmata of three bromeliad species (Aechmea gamosepala, Vriesea friburgensis, and Vriesea platynema) at a single Atlantic Forest site in southern Brazil. We sampled multiple individuals per species and multiple tanks from each individual, totalizing 30 samples. We observed very high levels of diversity in these communities, and remarkable variation across individuals and even among tanks from the same individual. The alpha diversity was higher for prokaryotes than eukaryotes, especially for A. gamosepala and V. platynema samples. Some biotic components appeared to be species-specific, while most of the biota was shared among species, but varied substantially in frequency among samples. Interestingly, V. friburgensis communities (which were sampled at nearby locations) tended to be more heterogeneous across samples, for both eukaryotes and prokaryotes. The opposite was true for V. platynema, whose samples were more broadly spaced but whose communities were more similar to each other. Our results indicate that additional attention should be devoted to within-individual heterogeneity when assessing bromeliad phytotelmata biodiversity, and highlight the complexity of the biotic assemblages gathered in these unique habitats. © 2020 International Society of Protistologists.

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