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Reliability of quantitating proliferative cell nuclear antigen in epithelial ovarian tumors.

Authors
Type
Published Article
Journal
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology
Publication Date
Volume
16
Issue
2
Pages
85–89
Identifiers
PMID: 7913806
Source
Medline
License
Unknown

Abstract

Sources of variability in quantitating proliferative cell nuclear antigen (PCNA) by image analysis were evaluated in paraffin sections of 18 ovarian tumors of low malignant potential (LMP) and grade 1 (G1) carcinomas. The correlation coefficient of reliability (R) was calculated to determine how reliable a single observation was for representing a "true" tumor value. Reliability of 61% was obtained when interobserver and intraobserver variability were assessed. Threshold settings for positive nuclear and antibody signals minimally affected the overall reliability. The reliability of a single block of tumor for representing true tumor value was R = 0.61. These sources of variability render this technique impractical for evaluating proliferative characteristics in LMP and G1 common epithelial ovarian tumors with PCNA staining.

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