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Release of liposomal markers by Cerebratulus toxin A-III.

Authors
  • Blumenthal, K M
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
May 31, 1984
Volume
121
Issue
1
Pages
14–18
Identifiers
PMID: 6375665
Source
Medline
License
Unknown

Abstract

Marker release from liposomes induced by the cytolytic protein Cerebratulus lacteus toxin A-III was studied. No phospholipid specificity was apparent, but the sensitivity of liposomes to A-III varied with the membrane fluidity. With dioleylphosphatidylcholine liposomes, complete release occurred at 10-20 micrograms toxin per ml, depending on marker size. Kinetic experiments showed that release was rapid and exhibited no lag phase. The diameter of the A-III produced membrane lesion must exceed 90 A, as tetrameric Concanavalin A is quantitatively released from A-III treated liposomes.

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