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Release of extracellular purines from plant roots and effect on ion fluxes.

Authors
Type
Published Article
Journal
Plant Signaling & Behavior
1559-2324
Publisher
Landes Bioscience
Publication Date
Volume
6
Issue
11
Pages
1855–1857
Identifiers
DOI: 10.4161/psb.6.11.17014
PMID: 22057319
Source
Medline
License
Unknown

Abstract

Extracellular purine nucleotides appear capable of regulating plant development, defence and stress responses by acting in part as agonists of plasma membrane calcium channels. Factors stimulating ATP release include wounding, osmotic stress and elicitors. Here we show that exogenous abscisic acid and L-glutamate can also cause ATP accumulation around Arabidopsis thaliana roots. Release of ADP from root epidermis would trigger ionotropic receptor-like activity in the plasma membrane, resulting in transient elevation of cytosolic free calcium. Root epidermal protoplasts (expressing aequorin as a cytosolic free calcium reporter) can support an extracellular ADP-induced cytosolic calcium elevation in the presence of an extracellular reductant. This confirms that ADP could elicit calcium-based responses distinct to those of ATP, which have been shown previously to involve production of extracellular reactive oxygen species.

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