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Release of dopamine from mesencephalic neurons in aggregate cultures: influence of target and non-target cells.

Authors
Type
Published Article
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
307
Issue
1-2
Pages
347–350
Identifiers
PMID: 6467004
Source
Medline
License
Unknown

Abstract

Spontaneous release of [3H]dopamine (DA) was observed from reaggregates of dissociated cells from fetal rostral mesencephalic tegmentum (RMT) containing DA neurons cocultured with their axonal target cells from striatum (CS) or frontal cortex (FCx), or with non-target cells from occipital cortex (OCx), or tectum. Such release increased in response to 50 mM K+. Tetrodotoxin (TTX) suppressed the spontaneous release from RMT-CS and RMT-FCx reaggregates by 42%; from RMT-tectum reaggregates by 24%, and did not significantly inhibit the release from RMT-OCx cocultures. Since TTX blocks spontaneous neuronal activity, these results suggest that the presence of axonal target cells enhances the activity of the dopamine neurons. DA neurons within RMT-FCx reaggregates released significantly more [3H]DA in response to 50 mM K+ than in RMT-CS cocultures. This result is in accord with the findings in vivo that inhibitory feedback mechanisms on DA release, present in the striatum, are lacking in the frontal cortex.

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