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Relationship of foetal number and parity in Barbari goats to plasma profile of caprine pregnancy-associated glycoprotein (caPAG) during gestation and the early postpartum period.

Authors
  • Singh, S P1
  • Ramachandran, N2
  • Sharma, N2
  • Goel, A K2
  • de Sousa, N M3
  • Beckers, J F3
  • Swain, D K4
  • Singh, M K5
  • Kharche, S D2
  • 1 Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India. Electronic address: [email protected] , (India)
  • 2 Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India. , (India)
  • 3 Laboratory of Animal Endocrinology and Reproduction, Faculty of Veterinary Medicine, University of Liege, 4000, Belgium. , (Belgium)
  • 4 Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India. , (India)
  • 5 Animal Genetics and Breeding Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India. , (India)
Type
Published Article
Journal
Animal reproduction science
Publication Date
Nov 01, 2019
Volume
210
Pages
106190–106190
Identifiers
DOI: 10.1016/j.anireprosci.2019.106190
PMID: 31635784
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study was conducted to characterise pregnancy-associated glycoprotein (caPAG) in peripheral plasma during gestation and postpartum periods of nulliparous and multiparous does with one or two foetuses using a caPAG specific two-step sandwich ELISA system. Earliest time-points for detection of pregnancy and foetal number with appropriate cut-off values were identified. Plasma samples from 15 pregnant (multiparous: n = 8; nulliparous: n = 7; during pregnancy and postpartum period) and six non-pregnant (during oestrous cycle) goats were collected and analysed. Mean caPAG concentration was greater than the threshold for pregnancy detection (S-N = 0.40) on d22, peaked on d45 and remained unchanged until parturition. From d45 until parturition, caPAG concentration in multiparous does with two foetuses was 1.4 to 1.8 fold greater (P < 0.001) than those with one foetus. For the ELISA, 0.83 (S-N) was the most appropriate cut-off to differentiate does with two from those with a single foetus with an overall sensitivity and accuracy of 88.9% and 84.7%, respectively. Circulating caPAG concentration in multiparous goats was greater (P < 0.05) compared with nulliparous goats during the early pregnancy and postpartum periods. After parturition, caPAG concentrations markedly decreased and were basal within 14 days postpartum. In conclusion, using the caPAG specific ELISA, results indicated there were unique gestational and postpartum profiles for caPAG concentrations that are affected by number of foetuses and parity of the doe. The marked decrease in concentration of caPAG following parturition indicates there would not be compromising of the detection of subsequent pregnancies in goats using this technique. Copyright © 2019 Elsevier B.V. All rights reserved.

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