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Relationship between the methionine tryptic peptides of simian virus 40 and BK virus tumor antigens.

Authors
  • Simmons, D T
  • Takemoto, K K
  • Martin, M A
Type
Published Article
Journal
Journal of virology
Publication Date
Oct 01, 1977
Volume
24
Issue
1
Pages
319–325
Identifiers
PMID: 198582
Source
Medline
License
Unknown

Abstract

The monomer form of BK virus (BKV) tumor antigen (T Ag) was immunoprecipitated from extracts of BKV-transformed cells and had a molecular weight of approximately 113,000. This compared with 97,000 for the molecular weight of either BKV or simian virus 40 (SV40) T Ag from lytically infected cells. The SV40 and BKV T Ag's from productively infected cells were compared by examining their methionine-labeled tryptic peptides. Out of a total of 20 SV40-and 21 BKV-specific peptides, there were seven pairs of similar peptides on the basis of ion-exchange chromatography, These coeluting peptides contained approximately 25 to 30% of the total methionine radioactivity. Similar results were obtained when the tryptic peptides of SV40 T Ag from lytically infected cells were compared with those of BKV T Ag from virally transformed cells.

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