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Regulation of SNAREs by tomosyn and ROCK: implication in extension and retraction of neurites.

Authors
  • Sakisaka, Toshiaki
  • Baba, Takeshi
  • Tanaka, Shintaro
  • Izumi, Genkichi
  • Yasumi, Masato
  • Takai, Yoshimi
Type
Published Article
Journal
The Journal of cell biology
Publication Date
Jul 05, 2004
Volume
166
Issue
1
Pages
17–25
Identifiers
PMID: 15240567
Source
Medline
License
Unknown

Abstract

Extension of neurites requires the SNARE-dependent fusion of plasmalemmal precursor vesicles with the plasma membrane of growth cones. Here, we show that tomosyn localizes at the palm of growth cones and inhibits the fusion of the vesicles there, thus promoting transport of the vesicles to the plasma membrane of the leading edges of growth cones. Tomosyn localizes because ROCK activated by Rho small G protein phosphorylates syntaxin-1, which increases the affinity of syntaxin-1 for tomosyn and forms a stable complex with tomosyn, resulting in inhibition of the formation of the SNARE complex. In retraction of neurites, tomosyn localizes all over the edges of the neurites and inhibits fusion of the vesicles with the plasma membrane. Thus, tomosyn demarcates the plasma membrane by binding to syntaxin-1 phosphorylated by ROCK, and thereby regulates extension and retraction of neurites.

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