Regulation of Nonylphenol-Induced Reproductive Toxicity in Mouse Spermatogonia Cells by miR-361-3p.
Department of Urology, Baoji People's Hospital, Baoji 721000, Shananxi, China.
Department of Urology, Xijing hospital, the Fourth Military Medical University, Xi'an 710032, Shananxi, China.
- Published Article
Molecular Reproduction and Development
Wiley (John Wiley & Sons)
- Publication Date
Oct 12, 2017
Nonylphenol (NP) is an environmental chemical that affects apoptosis and male infertility. In our study, we found that a high concentration of NP could down-regulate the expression of microRNA-361-3p (miR-361-3p) in the murine GC-1 spermatogonia cell line and in vivo in murine spermatogonia. Additionally, one direct target of this miR, the 3' untranslated region of Killin (Klln) mRNA, was identified. Klln encodes a transcription factor that directly regulates the expression of Tp73 (transcriptionally active p73), whose encoded protein can up-regulate the expression of Puma (P53 upregulated modulator of apoptosis). Thus, our investigation revealed that the expression of Klln, Tp73, and Puma increased upon NP-dependent down-regulation of miR-361-3p, which eventually leads to apoptosis of spermatogonia. This article is protected by copyright. All rights reserved.
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This record was last updated on 06/09/2018 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/29024157