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Regulation of nonylphenol-induced reproductive toxicity in mouse spermatogonia cells by miR-361-3p.

Authors
  • Tang, Liyan1
  • Zhao, Bo1
  • Zhang, Hui1
  • Du, Qiao1
  • Zhu, Jiang1
  • Zhao, Zhijiang1
  • Chen, Ce1
  • Luo, Cheng1
  • Kang, Qiyuan1
  • Yuan, Wenbing1
  • Bian, Shaohua1
  • Bi, Hang1
  • Sun, Huimin2
  • Li, Yingyi1
  • 1 Department of Urology, Baoji People's Hospital, Baoji, Shananxi, China. , (China)
  • 2 Department of Urology, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shananxi, China. , (China)
Type
Published Article
Journal
Molecular Reproduction and Development
Publisher
Wiley (John Wiley & Sons)
Publication Date
Dec 01, 2017
Volume
84
Issue
12
Pages
1257–1270
Identifiers
DOI: 10.1002/mrd.22923
PMID: 29024157
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nonylphenol (NP) is an environmental chemical that affects apoptosis and male infertility. In our study, we found that a high concentration of NP could down-regulate the expression of microRNA-361-3p (miR-361-3p) in the murine GC-1 spermatogonia cell line and in vivo in murine spermatogonia. Additionally, one direct target of this miR, the 3' untranslated region of Killin (Klln) mRNA, was identified. Klln encodes a transcription factor that directly regulates the expression of Tp73 (transcriptionally active p73), whose encoded protein can up-regulate the expression of Puma (p53 upregulated modulator of apoptosis). Thus, our investigation revealed that the expression of Klln, Tp73, and Puma increased upon NP-dependent down-regulation of miR-361-3p, which eventually leads to apoptosis of spermatogonia. © 2017 Wiley Periodicals, Inc.

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