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Regulation of neurotransmitter synthesis: from neuron to gene.

Authors
  • Bernard, C
  • Chireux, M
  • Barraille, P
  • Levan Thaï, A
  • Vidal, S
  • Kitahama, K
  • Weber, M
Type
Published Article
Journal
Journal de physiologie
Publication Date
Jan 01, 1991
Volume
85
Issue
2
Pages
97–104
Identifiers
PMID: 1684618
Source
Medline
License
Unknown

Abstract

We are interested in the molecular mechanisms of the regulation of neurotransmitter related gene expression by neurotrophic factors and neuronal activity. Previous work has shown that conditioned medium of muscle cells induces choline acetyltransferase (CAT) activity and represses tyrosine hydroxylase, dopamine-beta-hydroxylase and aromatic L-amino acid decarboxylase (AADC) activities in cultured sympathetic neurons. Here, we show that a new muscle-derived cell line secretes two factors which induce CAT activity in spinal cord cultures; one of those is related to LIF, a CAT inducing factor for sympathetic neurons. Preliminary data are presented on the structure of the human AADC and CAT genes. Putative promoter regions have been coupled to reporter genes; transient transfection experiments will allow us to determine the promoter elements responsible for the regulation by neurotrophic factors. We also summarize the distribution of AADC-immunoreactive cells in rat and cat brain, which will be used as a reference for the study of the specificity of expression of AADC promoter in transgenic mice.

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