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Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells.

Authors
  • An, So-Young1
  • Lee, Miri1
  • Yoon, Hyun-Kyoung1
  • Abekura, Fukushi2
  • Kim, Kyoung-Sook1
  • Kim, Dong-Hyun1
  • Kim, Hyeon-Jun3
  • Lee, Kichoon4
  • Kim, Cheorl-Ho5
  • Lee, Young-Choon6
  • 1 Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea. , (North Korea)
  • 2 Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do, 16419, South Korea. , (North Korea)
  • 3 Department of Orthopaedic Surgery, College of Medicine, Dong-A University, Busan, 49201, South Korea. , (North Korea)
  • 4 Functional Genomics Laboratory, Department of Animal Sciences, the Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210, USA.
  • 5 Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do, 16419, South Korea. [email protected] , (North Korea)
  • 6 Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea. [email protected] , (North Korea)
Type
Published Article
Journal
Glycoconjugate Journal
Publisher
Springer-Verlag
Publication Date
Dec 01, 2020
Volume
37
Issue
6
Pages
681–690
Identifiers
DOI: 10.1007/s10719-020-09959-3
PMID: 33108606
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this study, we found that gene expression of the human β-galactoside α2,6-sialyltransferase (hST6Gal I) was specifically increased during differentiation of human MG-63 osteoblastic cells by serum starvation (SS). In parallel, a distinct increase in binding to SNA, the α2,6-sialyl-specific lectin, was observed in serum-starved cells, as demonstrated by FACS analysis. 5'-Rapid amplification of cDNA ends analysis demonstrated that the increase of hST6Gal I transcript by SS is mediated by P1 promoter. To elucidate transcriptional regulation of hST6Gal I in SS-induced MG-63 cells, we functionally characterized the P1 promoter region of the hST6Gal I gene. The 5'-deletion analysis of P1 promoter region revealed that the 189 bp upstream region of transcription start site is critical for transcriptional activity of hST6Gal I gene in SS-induced MG-63 cells. This region contains the predicted binding sites for several transcription factors, including AREB6, FOXP1, SIX3, HNF1, YY2, and MOK2. The mutagenesis analysis for these sites and chromatin immunoprecipitation assay demonstrated that the YY2 binding site at -98 to -77 was essential for the SS-induced hST6Gal I gene expression during differentiation of MG-63 cells.

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