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Regulation of cyclin E gene expression by the human papillomavirus type 16 E7 oncoprotein.

Authors
Type
Published Article
Journal
The Journal of general virology
Publication Date
Volume
80 ( Pt 8)
Pages
2103–2113
Identifiers
PMID: 10466809
Source
Medline
License
Unknown

Abstract

In this study, we characterized the 5' regulatory region of the murine cyclin E gene and analysed activation of the gene by the E7 oncogene of human papillomavirus type 16 in transfection experiments. We found that the murine cyclin E promoter is composed of multiple regulatory elements, and we present evidence for at least two independent transcription units, designated P1 and P2. Overlapping binding sites for the cellular transcription factors Sp1 and E2F were identified in both promoters, and we found that E2F-mediated activation of transcription is inhibited by Sp1 in cotransfection experiments. The E2F/Sp1 binding sites contribute to transcriptional activation by E7, and the data suggest that the cyclin E gene is rendered E7-inducible through the combination of several cis-acting elements which display only weak intrinsic responsiveness to E7.

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