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Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model

Authors
  • Ahmad, F.1
  • Soe, S.1
  • White, N.2
  • Johnston, R.3
  • Khan, I.4
  • Liao, J.5, 6
  • Jones, M.1
  • Prabhu, R.6
  • Maconochie, I.7
  • Theobald, Peter1
  • 1 Cardiff University, School of Engineering, Cardiff, UK , Cardiff (United Kingdom)
  • 2 Cardiff University, School of Optometry and Vision Sciences, Cardiff, UK , Cardiff (United Kingdom)
  • 3 Swansea University, College of Engineering, Swansea, UK , Swansea (United Kingdom)
  • 4 Swansea University, Swansea University Medical School, Swansea, UK , Swansea (United Kingdom)
  • 5 The University of Texas at Arlington, Department of Bioengineering, Arlington, USA , Arlington (United States)
  • 6 Mississippi State University, Department of Biological Engineering, Centre for Advanced Vehicular Systems, Mississippi, USA , Mississippi (United States)
  • 7 Imperial College NHS Healthcare Trust, London, UK , London (United Kingdom)
Type
Published Article
Journal
Annals of Biomedical Engineering
Publisher
Springer-Verlag
Publication Date
Jul 16, 2018
Volume
46
Issue
12
Pages
2162–2176
Identifiers
DOI: 10.1007/s10439-018-2089-4
Source
Springer Nature
Keywords
License
Green

Abstract

The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload. In some cases, however, the heart structure does not fully develop causing poor circulation and inefficient oxygenation, which is associated with an increase in mortality and morbidity. This study focuses on developing an enhanced knowledge of the 1-day old heart, quantifying the region-specific microstructural parameters of the tissue. This will enable more accurate mathematical and computational simulations of the young heart. Hearts were dissected from 12, 1-day-old deceased Yorkshire piglets (mass: 2.1–2.4 kg, length: 0.38–0.51 m), acquired from a breeding farm. Evans blue dye was used to label the heart equator and to demarcate the left and right ventricle free walls. Two hearts were used for three-dimensional diffusion-tensor magnetic resonance imaging, to quantify the fractional anisotropy (FA). The remaining hearts were used for two-photon excited fluorescence and second-harmonic generation microscopy, to quantify the cardiomyocyte and collagen fibril structures within the anterior and posterior aspects of the right and left ventricles. FA varied significantly across both ventricles, with the greatest in the equatorial region, followed by the base and apex. The FA in each right ventricular region was statistically greater than that in the left. Cardiomyocyte and collagen fibre rotation was greatest in the anterior wall of both ventricles, with less dispersion when compared to the posterior walls. In defining these key parameters, this study provides a valuable insight into the 1-day-old heart that will provide a valuable platform for further investigation the normal and abnormal heart using mathematical and computational models.

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