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Regarding the measurement of microviscosity in lipid bilayers by EPR.

Authors
  • Gennaro, A M1
  • 1 Instituto de Física del Litoral, IFIS Litoral, CONICET-UNL, Güemes 3450, 3000 Santa Fe, Argentina. Electronic address: [email protected] , (Argentina)
Type
Published Article
Journal
Biophysical chemistry
Publication Date
Sep 01, 2019
Volume
252
Pages
106223–106223
Identifiers
DOI: 10.1016/j.bpc.2019.106223
PMID: 31325894
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Simulations of electron paramagnetic resonance spectra of stearic acid spin labels in lipid bilayers are used to illustrate the fact that the apparent order parameter Sapp calculated from the spectral shape does not coincide with the true order parameter S in the case of slow motions. While S reflects a static property as the degree of order of the lipid chains, Sapp depends on both order and dynamics. Thus, calibration procedures intended to obtain bilayer microviscosity values from Sapp in the slow motion regime are not reliable. However, Sapp is a useful tool to describe trends in membrane fluidity. Copyright © 2019 Elsevier B.V. All rights reserved.

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