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Reference intervals, intraindividual and interindividual variability, and reference change values for hematologic variables in laboratory beagles.

Authors
  • Bourgès-Abella, Nathalie H1
  • Gury, Thierry D2
  • Geffré, Anne3
  • Concordet, Didier4
  • Thibault-Duprey, Kevin C2
  • Dauchy, Arnaud2
  • Trumel, Catherine3
  • 1 Université de Toulouse, Université Paul Sabatier (UPS), Institut National Polytechnique (INP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Unité Mixte de Service (UMS) 006, Laboratoire Central de Biologie Médicale, Toulouse, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France. [email protected] , (France)
  • 2 SANOFI-AVENTIS Recherche & Développement, Groupe SANOFI, Disposition, Safety and Animal Research, Alfortville, France. , (France)
  • 3 Université de Toulouse, Université Paul Sabatier (UPS), Institut National Polytechnique (INP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Unité Mixte de Service (UMS) 006, Laboratoire Central de Biologie Médicale, Toulouse, France, Institut National de la Santé et de la Recherche Médicale (INSERM), UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France. , (France)
  • 4 Université de Toulouse, INP, ENVT, Unité Mixte de Recherche (UMR) 1331 Toxalim, Toulouse, France. , (France)
Type
Published Article
Journal
Journal of the American Association for Laboratory Animal Science : JAALAS
Publication Date
Jan 01, 2015
Volume
54
Issue
1
Pages
17–24
Identifiers
PMID: 25651086
Source
Medline
License
Unknown

Abstract

In research and development studies for human and veterinary medicine, relevant comparators for interpreting clinical pathology results are matched with concurrent control animals. However, reference intervals (RI) provide a comparator database and important aids for interpreting clinical pathology data, especially in laboratory beagle dogs. Furthermore, RI incorporate biologic variation, which includes analytical, intraindividual, and interindividual variation. No studies to date have established RI and studied the effect of biologic variation on hematologic variables in a large group of laboratory dogs. The purpose of this retrospective study was to establish hematologic RI for laboratory beagles according to international recommendations and estimate the effect of biologic variation in routinely measured hematologic analytes by using the databank at a pharmaceutical center. Blood specimens from 340 healthy beagles (age, 9 to 36 mo) were evaluated by using a flow-cytometry-based hematology analyzer. RI and their 90% confidence intervals were established by using a nonparametric method. Effects of sex, age, and weight were investigated. Weight had no effect on any analyte. RBC, Hgb, Hct, MCV, MCH, RBC distribution width, and platelet count increased with age, whereas WBC count decreased. The only clinically relevant effect of sex was observed for platelets, which were lower in male beagles than in female and warranted 2 different RI. The calculated index of individuality showed that population-based RI were appropriate for almost all hematologic analytes, as might be expected for a homogeneous group of laboratory beagles.

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