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Re-evaluating microglia expression profiles using RiboTag and cell isolation strategies

Authors
  • Haimon, Zhana1
  • Volaski, Alon1
  • Orthgiess, Johannes2
  • Boura-Halfon, Sigalit1
  • Varol, Diana1
  • Shemer, Anat1
  • Yona, Simon1
  • Zuckerman, Binyamin3
  • David, Eyal1
  • Chappell-Maor, Louise1
  • Bechmann, Ingo4
  • Gericke, Martin4
  • Ulitsky, Igor3
  • Jung, Steffen1
  • 1 Weizmann Institute of Science, Department of Immunology, Rehovot, Israel , Rehovot (Israel)
  • 2 University of Leipzig, Carl Ludwig Institute of Physiology, Leipzig, Germany , Leipzig (Germany)
  • 3 Weizmann Institute of Science, Department of Biological Regulation, Rehovot, Israel , Rehovot (Israel)
  • 4 University of Leipzig, Institute of Anatomy, Leipzig, Germany , Leipzig (Germany)
Type
Published Article
Journal
Nature Immunology
Publisher
Springer Nature
Publication Date
May 18, 2018
Volume
19
Issue
6
Pages
636–644
Identifiers
DOI: 10.1038/s41590-018-0110-6
Source
Springer Nature
License
Yellow

Abstract

Isolating tissue-resident cells runs the risk of altering their expression profile. Jung and colleagues use a RiboTagging approach to describe the microglial translatome and demonstrate that macrophage extraction from their tissue environment results in significant transcriptional alterations.

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