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Reduced susceptibility to a tobacco bushy top virus Malawi isolate by loss of function in host eIF(iso)4E genes.

Authors
  • Udagawa, Hisashi1, 2
  • Koga, Kazuharu1
  • Shinjo, Akira1
  • Kitashiba, Hiroyasu2
  • Takakura, Yoshimitsu1
  • 1 Leaf Tobacco Research Center, Japan Tobacco, Inc., 1900, Idei, Oyama, Tochigi 323-0808, Japan. , (Japan)
  • 2 Graduate School of Agricultural Science, Tohoku University, 468-1, Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-0845, Japan. , (Japan)
Type
Published Article
Journal
Breeding science
Publication Date
Jun 01, 2020
Volume
70
Issue
3
Pages
313–320
Identifiers
DOI: 10.1270/jsbbs.19135
PMID: 32714053
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Tobacco bushy top disease (TBTD) is a viral disease of tobacco (Nicotiana tabacum L.) caused by mixed infection of Tobacco bushy top virus or Ethiopian tobacco bushy top virus and a helper virus. Despite its damage to tobacco, practical genetic resources for disease resistance have not been found. Here, we report that a mutation of tobacco eIF(iso)4E genes (eIF(iso)4E-S and eIF(iso)4E-T), which encode eukaryotic translation initiation factors, confers resistance (reduced susceptibility) to TBTD caused by a virus from Malawi (designated as tobacco bushy top virus Malawi isolate, TBTV-MW). RNAi lines in which eIF(iso)4E genes were silenced showed reduced susceptibility to TBTV-MW. We also tested chemically-induced single (eIF(iso)4E-S or eIF(iso)4E-T) and double (eIF(iso)4E-S and eIF(iso)4E-T) nonsense mutants for resistance to TBTV-MW. Suppression of eIF(iso)4E-S showed reduced susceptibility, and the resistance of the double mutant tended to be even stronger. eIF(iso)4E mutants also showed reduced susceptibility to TBTV-MW transmitted by aphids. To the best of our knowledge, the eIF(iso)4E-S mutant is the first genetic resource for TBTD resistance breeding in tobacco. Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.

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