Affordable Access

Reduced levels of DEAD-box proteins DBP-RB and p72 in fetal Down syndrome brains.

Authors
  • Kircher, Susanne G
  • Kim, Seong Hwan
  • Fountoulakis, Michael
  • Lubec, Gert
Type
Published Article
Journal
Neurochemical research
Publication Date
Oct 01, 2002
Volume
27
Issue
10
Pages
1141–1146
Identifiers
PMID: 12462412
Source
Medline
License
Unknown

Abstract

Down syndrome (DS) is characterized by abnormal brain morphology and neurological and behavioral functions. The pivotal role of helicases in brain development, growth, and differentiation made us evaluate three DEAD BOX proteins, DEAD-box protein 1 (DBP-RB), DEAD-box protein 3 (HLP2), DEAD-box protein 72 (P72), and the RuvB-like DNA helicase (TIP49b), in fetal brain of controls and DS subjects, using two-dimensional electrophoresis with subsequent mass spectroscopic (MALDI-MS) identification. HLP2 and TIP49b brain levels were comparable between DS and controls, and protein levels of p72 and DBP-RB were significantly reduced in DS fetal cortex (p72: 2.04+/-1.90 vs. 5.57+/-2.56 in controls, p < 0.01; DBP-RB: 0.58+/-0.94 vs. 1.90+/-0.97 in controls, p < 0.01). Impairment of the helicases p72 and DBP-RB may reflect or lead to deficient growth and differentiation of brain development early in life and can be considered pathogenetic factors along with the reported deficits of transcription, splicing, and elongation factors already described in fetal DS brains.

Report this publication

Statistics

Seen <100 times