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Recovery response of dividing cells in the thymus of whole-body gamma-irradiated mice.

Authors
  • Suciu, D
  • Uray, Z
  • Maniu, M
Type
Published Article
Journal
International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine
Publisher
Informa UK (Taylor & Francis)
Publication Date
Nov 01, 1976
Volume
30
Issue
5
Pages
409–417
Identifiers
PMID: 1087284
Source
Medline
License
Unknown

Abstract

Mice were irradiated with different doses of gamma-rays 30 min after the administration of 32P-orthophosphate. The dose-response curves determined at 72 hours after exposure showed an inflection point in the total activity present in the DNA in thymus and spleen. In the low dose-range, the dose-response curves have D0 = 55 rad (n = 2-5) for thymus and DO = 95 rad (n = 2-5) for the spleen. Thirty minutes after the administration of 32P-orthophosphate, the dividing cells from thymus were partially synchronized by the administration of 80 mg per kg body-weight hydroxyurea. At different time-intervals, the mice were irradiated with 80 rad, and the total activity of DNA was determined at 72 hours after synchronization. A significant maximum of recovery was found at 5 hours (S phase) after the administration of hydroxyurea. In similar conditions, the dose-response curves corresponding to the G1, S and M phase of the division cycle were also determined. The synchronization of dividing cells induced by hydroxyurea failed in the spleen.

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