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Recovery of DNA from agarose gels using a modified Elutrap.

Authors
  • Gu, H1
  • Wilson, D
  • Inselburg, J
  • 1 Division of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208.
Type
Published Article
Journal
Journal of Biochemical and Biophysical Methods
Publisher
Elsevier
Publication Date
Mar 01, 1992
Volume
24
Issue
1-2
Pages
45–50
Identifiers
PMID: 1560180
Source
Medline
License
Unknown

Abstract

We have made a significant improvement in the electroelution device, Elutrap (Schleicher and Schuell) by substituting an agarose gel barrier, which is made from 0.6% agarose (SeaKem GTG; FMC Corporation), into the elution chamber in place of the manufacturer specified BT2 membrane. This modification substantially increases the DNA recovery from agarose gels, even in samples containing less than 1 microgram of DNA, and shortens elution times particularly for large sizes of DNA (greater than 4.4 kbp). Additionally, the gel barrier provides a reproducible quantity and quality of DNA recovery. The high quality of the eluted DNA using the modified Elutrap makes this system suitable for further DNA manipulations.

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