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Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics

Authors
  • González-Domínguez, Raúl1, 2
  • González-Domínguez, Álvaro3
  • Sayago, Ana1, 2
  • Fernández-Recamales, Ángeles1, 2
  • 1 (Á.F.-R.)
  • 2 International Campus of Excellence CeiA3, University of Huelva, 21007 Huelva, Spain
  • 3 Institute of Research and Innovation in Biomedical Sciences of the Province of Cádiz (INiBICA), 11009 Cádiz, Spain
Type
Published Article
Journal
Metabolites
Publisher
MDPI
Publication Date
Jun 03, 2020
Volume
10
Issue
6
Identifiers
DOI: 10.3390/metabo10060229
PMID: 32503183
PMCID: PMC7344701
Source
PubMed Central
Keywords
License
Green

Abstract

Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample variability driven by these technical issues, as well as for maintaining the metabolic integrity of biological samples to ensure that metabolomic profiles are a direct expression of the in vivo biochemical status. This review article provides an updated literature revision of the most important factors related to sample handling and pre-processing that may affect metabolomics results, particularly focusing on the most commonly investigated biofluids in metabolomics, namely blood plasma/serum and urine. Finally, we also provide some general recommendations and best practices aimed to standardize and accurately report all these pre-analytical aspects in metabolomics research.

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