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Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1.

Authors
  • Molinková, D
  • Celer, V
Type
Published Article
Journal
Folia microbiologica
Publication Date
Jan 01, 2006
Volume
51
Issue
5
Pages
492–496
Identifiers
PMID: 17176773
Source
Medline
License
Unknown

Abstract

Single chain Fv (scFv) molecules generated by phage-display technology represent a new and efficient tool in the research and diagnostics of infectious diseases. The recombinant glycoprotein D of Equid herpesvirus 1 was successfully expressed in E. coli cells. The protein was produced predominantly in soluble fraction and was then purified on a nickel-agarose column. The scFv antibodies against the glycoprotein were selected and several clones of glycoprotein D-specific scFv-antibodies were identified; t of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and used as reagent in an immunofluorescence test.

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