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The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties

Authors
  • Gorokhovatsky, A. Yu.1
  • Chepurnykh, T. V.1
  • Shcheglov, A. S.1
  • Mokrushina, Yu. A.1
  • Baranova, M. N.1
  • Goncharuk, S. A.1
  • Purtov, K. V.2
  • Petushkov, V. N.2
  • Rodionova, N. S.2
  • Yampolsky, I. V.1
  • 1 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia , Moscow (Russia)
  • 2 Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk, Russia , Krasnoyarsk (Russia)
Type
Published Article
Journal
Doklady Biochemistry and Biophysics
Publisher
Pleiades Publishing
Publication Date
Mar 10, 2021
Volume
496
Issue
1
Pages
52–55
Identifiers
DOI: 10.1134/S1607672921010051
Source
Springer Nature
Keywords
License
Green

Abstract

AbstractA key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.

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