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Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli.

Authors
  • Fong, Mun-Yik
  • Lau, Yee-Ling
  • Init, Ithoi
  • Jamaiah, I
  • Anuar, A Khairul
  • Rahmah, N
Type
Published Article
Journal
The Southeast Asian journal of tropical medicine and public health
Publication Date
Dec 01, 2003
Volume
34
Issue
4
Pages
723–726
Identifiers
PMID: 15115078
Source
Medline
License
Unknown

Abstract

The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

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