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Recognition of Lipoproteins by Toll-like Receptor 2 and DNA by the AIM2 Inflammasome Is Responsible for Production of Interleukin-1β by Virulent Suilysin-Negative Streptococcus suis Serotype 2

Authors
  • Lavagna, Agustina1
  • Auger, Jean-Philippe1
  • Giradin, Stephen E.
  • Gisch, Nicolas
  • Segura, Mariela1
  • Gottschalk, Marcelo1
  • 1 (M.S.)
Type
Published Article
Journal
Pathogens
Publisher
MDPI
Publication Date
Feb 21, 2020
Volume
9
Issue
2
Identifiers
DOI: 10.3390/pathogens9020147
PMID: 32098284
PMCID: PMC7168628
Source
PubMed Central
Keywords
License
Green

Abstract

Streptococcus suis serotype 2 is an important porcine bacterial pathogen and zoonotic agent causing sudden death, septic shock and meningitis. These pathologies are the consequence of an exacerbated inflammatory response composed of various mediators including interleukin (IL)-1β. Elevated levels of the toxin suilysin (SLY) were demonstrated to play a key role in S. suis -induced IL-1β production. However, 95% of serotype 2 strains isolated from diseased pigs in North America, many of which are virulent, do not produce SLY. In this study, we demonstrated that SLY-negative S. suis induces elevated levels of IL-1β in systemic organs, with dendritic cells contributing to this production. SLY-negative S. suis -induced IL-1β production requires MyD88 and TLR2 following recognition of lipoproteins. However, the higher internalization rate of the SLY-negative strain results in intracellularly located DNA being recognized by the AIM2 inflammasome, which promotes IL-1β production. Finally, the role of IL-1 in host survival during the S. suis systemic infection is beneficial and conserved, regardless of SLY production, via modulation of the inflammation required to control bacterial burden. In conclusion, this study demonstrates that SLY is not required for S. suis -induced IL-1β production.

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