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Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases.

Authors
  • Zielonka, Jacek1
  • Hardy, Micael2
  • Michalski, Radosław3
  • Sikora, Adam3
  • Zielonka, Monika4
  • Cheng, Gang4
  • Ouari, Olivier2
  • Podsiadły, Radosław5
  • Kalyanaraman, Balaraman4
  • 1 Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA. [email protected]
  • 2 Aix Marseille Univ, CNRS, ICR, 13013, Marseille, France. , (France)
  • 3 Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland. , (Poland)
  • 4 Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
  • 5 Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland. , (Poland)
Type
Published Article
Journal
Cell Biochemistry and Biophysics
Publisher
Springer-Verlag
Publication Date
Dec 01, 2017
Volume
75
Issue
3-4
Pages
335–349
Identifiers
DOI: 10.1007/s12013-017-0813-6
PMID: 28660426
Source
Medline
Keywords
License
Unknown

Abstract

NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases. Here, we describe the development of new assays for measuring the activity of NADPH oxidases enabling the high-throughput screening for NADPH oxidase inhibitors.

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