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Recent advances in high-throughput approaches to dissect enhancer function.

Authors
  • Santiago-Algarra, David1
  • Dao, Lan T M1
  • Pradel, Lydie1
  • España, Alexandre1
  • Spicuglia, Salvatore1
  • 1 Aix-Marseille University, TAGC, Marseille, France. , (France)
Type
Published Article
Journal
F1000Research
Publisher
"F1000 Research, Ltd."
Publication Date
Jan 01, 2017
Volume
6
Pages
939–939
Identifiers
DOI: 10.12688/f1000research.11581.1
PMID: 28690838
Source
Medline
Keywords
License
Unknown

Abstract

The regulation of gene transcription in higher eukaryotes is accomplished through the involvement of transcription start site (TSS)-proximal (promoters) and -distal (enhancers) regulatory elements. It is now well acknowledged that enhancer elements play an essential role during development and cell differentiation, while genetic alterations in these elements are a major cause of human disease. Many strategies have been developed to identify and characterize enhancers. Here, we discuss recent advances in high-throughput approaches to assess enhancer activity, from the well-established massively parallel reporter assays to the recent clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based technologies. We highlight how these approaches contribute toward a better understanding of enhancer function, eventually leading to the discovery of new types of regulatory sequences, and how the alteration of enhancers can affect transcriptional regulation.

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