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Recent advances in CE-mediated microanalysis for enzyme study.

Authors
  • Wang, Xu
  • Li, Kefeng
  • Adams, Erwin
  • Schepdael, Ann Van
Type
Published Article
Journal
Electrophoresis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2014
Volume
35
Issue
1
Pages
119–127
Identifiers
DOI: 10.1002/elps.201300294
PMID: 24170447
Source
Medline
Keywords
License
Unknown

Abstract

This review gives an overview of the recent developments and applications in the use of CE-mediated microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.

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