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Real-time measurements of milk fat globule membrane modulation during simulated intestinal digestion using electron paramagnetic resonance spectroscopy.

Authors
  • Alshehab, Maha1
  • Budamagunta, Madhu S2
  • Voss, John C2
  • Nitin, Nitin3
  • 1 Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, United States. , (United States)
  • 2 Department of Biochemistry and Molecular Medicine, University of California-Davis, Davis, CA 95616, United States. , (United States)
  • 3 Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Colloids and surfaces. B, Biointerfaces
Publication Date
Sep 26, 2019
Volume
184
Pages
110511–110511
Identifiers
DOI: 10.1016/j.colsurfb.2019.110511
PMID: 31600680
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Milk Fat Globules with their unique interfacial structure and membrane composition are a key nutritional source for mammalian infants, however, there is a limited understanding of the dynamics of fat digestion in these structures. Lipid digestion is an interfacial process involving interactions of enzymes and bile salts with the interface of suspended lipid droplets in an aqueous environment. In this study, we have developed an electron paramagnetic resonance spectroscopy approach to evaluate real time dynamics of milk fat globules interfacial structure during simulated intestinal digestion. To measure these dynamics, natural milk fat globule membrane was labeled with EPR-active probe, partitioning of EPR probes into MFGs membrane was validated using saturation-recovery measurements and calculation of the depth parameter Φ. After validation, the selected spin probe was used to evaluate the membrane's fluidity as a measure of the interface's modulation in the presence of bile salts and pancreatic lipase. Independently, bile salts were found to have a rigidifying effect on the spin probed MFGM, while pancreatic lipase resulted in an increase in membrane fluidity. When combined, the effect of lipase appears to be diminished in the presence of bile salts. These results indicate the efficacy of EPR in providing an insight into small time scale molecular dynamics of phospholipid interfaces in milk fat globules. Understanding interfacial dynamics of naturally occurring complex structures can significantly aid in understanding the role of interfacial composition and structural complexity in delivery of nutrients during digestion. Copyright © 2019 Elsevier B.V. All rights reserved.

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