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Real-Time and Label-Free Measurement of Deubiquitinase Activity with a MspA Nanopore

Authors
  • Shorkey, Spencer A.1
  • Du, Jiale2
  • Pham, Ryan2
  • Strieter, Eric R.1, 2
  • Chen, Min1, 2
  • 1 Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA 01003 (USA)
  • 2 Department of Chemistry, University of Massachusetts Amherst, Amherst, MA 01003 (USA)
Type
Published Article
Journal
ChemBioChem
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jun 16, 2021
Volume
22
Issue
17
Pages
2688–2692
Identifiers
DOI: 10.1002/cbic.202100092
PMID: 34060221
PMCID: PMC8416795
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

A MspA nanopore is demonstrated as a sensor for discriminating and quantifying various ubiquitin proteins. Real-time measurement of polymeric ubiquitin chain disassembly by a deubiquitinating enzyme was performed and enzyme kinetics were determined.

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