A real-time SUMO-binding assay for the analysis of the SUMO-SIM protein interaction network.
- Authors
- Type
- Published Article
- Journal
- Bioscience Biotechnology and Biochemistry
- Publisher
- Informa UK (Taylor & Francis)
- Publication Date
- Jan 01, 2010
- Volume
- 74
- Issue
- 6
- Pages
- 1302–1305
- Identifiers
- PMID: 20530889
- Source
- Medline
- License
- Unknown
Abstract
SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.
