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A real-time SUMO-binding assay for the analysis of the SUMO-SIM protein interaction network.

Authors
Type
Published Article
Journal
Bioscience Biotechnology and Biochemistry
1347-6947
Publisher
Informa UK (Taylor & Francis)
Publication Date
Volume
74
Issue
6
Pages
1302–1305
Identifiers
PMID: 20530889
Source
Medline
License
Unknown

Abstract

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

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