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Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Authors
  • Czapela, Fabiane Fernanda1
  • Kubeneck, Simone1
  • Preczeski, Karina Paula1
  • Dalastra, Caroline1
  • Scapini, Thamarys1
  • Bonatto, Charline1
  • Stefanski, Fábio Spitza1
  • Camargo, Aline Frumi1
  • Zanivan, Jessica1
  • Mossi, Altemir José1
  • Fongaro, Gislaine2
  • Treichel, Helen1
  • 1 Universidade Federal Da Fronteira Sul–UFFS, Rodovia ERS 135-Km 72, nº 200, Erechim, RS, 99700-970, Brazil , Erechim (Brazil)
  • 2 Universidade Federal de Florianópolis–UFSC, Engenheiro Agronômico Andrei Cristian Ferreira St., Trindade, Florianópolis, SC, 88040-900, Brazil , Florianópolis (Brazil)
Type
Published Article
Journal
Bioresources and Bioprocessing
Publisher
Springer Singapore
Publication Date
Sep 26, 2020
Volume
7
Issue
1
Identifiers
DOI: 10.1186/s40643-020-00338-2
Source
Springer Nature
Keywords
License
Green

Abstract

Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

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