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Reaction of vitamin K and dithiothreitol on reversed-phase C18 high-performance liquid chromatographic columns.

Authors
  • Canfield, L M
  • Holzman, R B
Type
Published Article
Journal
Journal of chromatography
Publication Date
Sep 07, 1984
Volume
299
Issue
1
Pages
225–231
Identifiers
PMID: 6490779
Source
Medline
License
Unknown

Abstract

A non-enzymatically catalyzed side reaction between dithiothreitol and vitamin K occurs on reversed-phase C18 high-performance liquid chromatographic columns. Excess dithiothreitol may be effectively removed from enzymatic reaction mixtures with N-ethylmaleimide. A low polarity gradient elution system is reported which gives quantitative recoveries (greater than 95%) of the vitamins in a concentration range useful for analysis of enzymatically catalyzed reactions.

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