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Rational design and implementation of a chemically inducible heterotrimerization system

Authors
  • Wu, Helen D.1, 1
  • Kikuchi, Masaki2
  • Dagliyan, Onur3
  • Aragaki, Adam K.1, 1
  • Nakamura, Hideki1, 4
  • Dokholyan, Nikolay V.5, 6
  • Umehara, Takashi2
  • Inoue, Takanari1, 1, 1
  • 1 Johns Hopkins University School of Medicine, Baltimore, MD, USA , Baltimore (United States)
  • 2 RIKEN Center for Biosystems Dynamics Research, Yokohama, Kanagawa, Japan , Yokohama (Japan)
  • 3 Harvard Medical School, Boston, MA, USA , Boston (United States)
  • 4 Kyoto University, Nishikyo-ku, Kyoto, Japan , Kyoto (Japan)
  • 5 Penn State University College of Medicine, Hershey, PA, USA , Hershey (United States)
  • 6 Pennsylvania State University, University Park, PA, USA , University Park (United States)
Type
Published Article
Journal
Nature Methods
Publisher
Springer Nature
Publication Date
Aug 03, 2020
Volume
17
Issue
9
Pages
928–936
Identifiers
DOI: 10.1038/s41592-020-0913-x
Source
Springer Nature
License
Yellow

Abstract

Chemically inducible trimerization tools based on split FRB or FKBP with full-length FKBP or FRB, respectively, expand the chemogenetics toolbox. Their efficiency and fast kinetics enable new types of protein manipulation in live cells.

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