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Rat adrenal chromaffin cells become sensitive to alpha-latrotoxin when cultured in vitro: the effect of nerve growth factor.

Authors
  • Kobayashi, H
  • Izumi, F
  • Meldolesi, J
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Mar 28, 1986
Volume
65
Issue
1
Pages
114–118
Identifiers
PMID: 3703377
Source
Medline
License
Unknown

Abstract

Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, alpha-latrotoxin (alpha-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to alpha-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to alpha-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

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