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[Rapid virus diagnosis by electrosyneresis (author's transl)].

Authors
  • Gaspar, A
  • Edlinger, E
Type
Published Article
Journal
Annales de microbiologie
Publication Date
Jan 01, 1978
Volume
129
Issue
4
Pages
545–552
Identifiers
PMID: 211893
Source
Medline
License
Unknown

Abstract

Electrosyneresis (counter-current immunoelectrophoresis) detected the presence of 7 coxsackieviruses and 1 poliovirus in 70 specimens of stools. Identical results were obtained by inoculation of mice and tissue cultures. First screening of specimens was undertaken with a human "standard" gammaglobulin; only specimens precipitating with this reagent contained viral antigens. Hence, especially in children, all negative stools could be excluded from subsequent tests with antiserum pools and monospecific antisera. In our experience, electrosyneresis is a specific method: every type of coxsackievirus and poliovirus types react only with the corresponding monospecific antisera. Electrosyneresis appears to be a sensitive procedure: viral antigens corresponding to 10(4) TCID50/ml can be dtected.

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