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Rapid, small scale purification of rice hoja blanca and Echinochloa hoja blanca tenuivirus ribonucleoprotein.

Authors
  • de Miranda, J R
  • Espinoza, A M
  • Hull, R
Type
Published Article
Journal
Journal of Virological Methods
Publisher
Elsevier
Publication Date
Jan 01, 1996
Volume
56
Issue
1
Pages
109–113
Identifiers
PMID: 8690758
Source
Medline
License
Unknown

Abstract

Highly purified tenuivirus ribonucleoprotein was obtained from small amounts of leaf tissue by sedimenting the ribonucleoprotein particles from debris-free plant extract into a 30% sucrose cushion, in 1.5-mL microfuge tubes. Using this protocol, significant size differences were discovered in the double-stranded forms of the viral genomic RNAs of rice hoja blanca tenuivirus and a tenuivirus isolated from Echinochloa colonum, a common weed of rice cultivation.

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