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Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings

Authors
  • Guselnikova, Olga
  • Dvorankova, Barbara
  • Kakisheva, Kamila
  • Kalachyova, Yevgeniya
  • Postnikov, Pavel
  • Svorcik, Vaclav
  • Lyutakov, Oleksiy
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
May 23, 2019
Volume
411
Issue
15
Pages
3309–3319
Identifiers
DOI: 10.1007/s00216-019-01801-6
Source
Springer Nature
Keywords
License
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Abstract

Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. Graphical abstract

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