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Rapid screening of colonies from Listeria selective agar.

Authors
  • Dealler, S F1
  • Rotowa, N A
  • 1 Department of Medical Microbiology, University of Leeds.
Type
Published Article
Journal
Journal of Hospital Infection
Publisher
Elsevier
Publication Date
February 1991
Volume
17
Issue
2
Pages
147–150
Identifiers
PMID: 1674261
Source
Medline
License
Unknown

Abstract

A rapid method ('Liststrip', Lab M) of screening aesculin-positive colonies from Oxford selective agar was evaluated. This method relies on the ability of all Listeria spp. to hydrolyse aesculin and to have a rapid phosphatase but neither a pyroglutamic acid beta-naphthylamide amidase nor produce indole. Of 198 Listeria spp., all gave identical results (as above) in 10 min (except four isolates of L. murrayi), whereas of 112 aesculin-positive colonies from Oxford agar that were not Listeria only one gave a similar result. The method was compared with Gram stain and oxidase as a screening procedure. This strip test method is simple enough to be used by relatively unskilled staff and provides a rapid and reliable method to screen colonies from Oxford selective agar.

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