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A rapid sample preparation method for the determination of sulfamethazine in swine tissues by high-performance liquid chromatography.

Authors
  • Haagsma, N
  • Nooteboom, R J
  • Gortemaker, B G
  • Maas, M J
Type
Published Article
Journal
Zeitschrift für Lebensmittel-Untersuchung und -Forschung
Publication Date
Sep 01, 1985
Volume
181
Issue
3
Pages
194–197
Identifiers
PMID: 4050127
Source
Medline
License
Unknown

Abstract

A rapid procedure for the determination of sulfamethazine in swine muscle and kidney is described. The method comprises sonication-aided extraction with dichloromethane, cleaning-up of the extract on a combination of silica and reversed-phase C18 Sep-Pak cartridges and analysis by high-performance liquid chromatography on a Hypersil ODS column using acetonitrile/ammonium acetate solution (10 mmol X 1(-1), pH 6.8) 1 + 3 v/v as the mobile phase. Detection was performed at 254 nm. Mean recoveries of added sulfamethazine from swine muscle and kidney, at levels of 0.05 to 0.5 micrograms X g-1, were 89.5 and 80.5%, respectively. After intramuscular injection of sulfamethazine into swine, residues in tissues were also determined. The application of ultrasonic energy for the extraction of sulfamethazine residues from animal tissues is also discussed.

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