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Rapid and robust optogenetic control of gene expression in Drosophila.

Authors
  • di Pietro, Florencia1
  • Herszterg, Sophie2
  • Huang, Anqi2
  • Bosveld, Floris1
  • Alexandre, Cyrille2
  • Sancéré, Lucas1
  • Pelletier, Stéphane1
  • Joudat, Amina1
  • Kapoor, Varun1
  • Vincent, Jean-Paul3
  • Bellaïche, Yohanns4
  • 1 Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France. , (France)
  • 2 Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK.
  • 3 Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK. Electronic address: [email protected]
  • 4 Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Developmental cell
Publication Date
Nov 29, 2021
Identifiers
DOI: 10.1016/j.devcel.2021.11.016
PMID: 34879263
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

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