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Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy.

Authors
  • Huang, Zufang
  • Sun, Yan
  • Wang, Jing
  • Du, Shengrong
  • Li, Yongzeng
  • Lin, Juqiang
  • Feng, Shangyuan
  • Lei, Jinping
  • Lin, Hongxin
  • Chen, Rong
  • Zeng, Haishan
Type
Published Article
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Publication Date
Dec 01, 2013
Volume
18
Issue
12
Pages
127003–127003
Identifiers
DOI: 10.1117/1.JBO.18.12.127003
PMID: 24343445
Source
Medline
License
Unknown

Abstract

In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.

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