Affordable Access

Access to the full text

Rapid Identification of OXA-48-like, KPC, NDM, and VIM Carbapenemase-Producing Enterobacteriaceae From Culture: Evaluation of the RESIST-4 O.K.N.V. Multiplex Lateral Flow Assay

Authors
  • Song, Wonkeun1
  • Park, Min-Jeong1
  • Jeong, Seri1
  • Shin, Dong Hoon1
  • Kim, Jae-Seok1
  • Kim, Hyun Soo1
  • Kim, Han-Sung1
  • Lee, Nuri1
  • Hong, Jun Sung1
  • Jeong, Seok Hoon1
  • 1 .
Type
Published Article
Journal
Annals of Laboratory Medicine
Publisher
The Korean Society for Laboratory Medicine
Publication Date
Dec 18, 2019
Volume
40
Issue
3
Pages
259–263
Identifiers
DOI: 10.3343/alm.2020.40.3.259
PMID: 31858767
PMCID: PMC6933055
Source
PubMed Central
Keywords
License
Green

Abstract

There is an urgent need for accurate and rapid diagnostic assays capable of identifying carbapenemase-producing Enterobacteriaceae (CPE). We assessed the performance of the RESIST-4 O.K.N.V. (OKNV) assay (Coris BioConcept, Gembloux, Belgium) for the identification of oxacillinase (OXA)-48-like-, Klebsiella pneumoniae carbapenemase (KPC)-, New Delhi metallo-β-lactamase (NDM)-, and Verona integron-encoded metallo-β-lactamase (VIM)-producing Enterobacteriaceae grown on sheep blood agar (SBA) and the CHROMagar KPC medium. Sixty-five carbapenem-resistant Enterobacteriaceae (CRE) isolates with characterized carbapenemase content were used to evaluate the OKNV assay. The assay correctly identified all 30 isolates that produced one of the four targeted carbapenemase families. Additionally, it correctly identified 15 isolates that co-produced KPC and NDM, VIM and NDM or OXA-48-like and NDM, but failed to identify an NDM-1 and OXA-232 co-producing Klebsiella pneumoniae isolate. All 16 non-carbapenemase-producing CRE and four CPE isolates exhibited negative results, and no cross-reaction was observed. Overall, the sensitivity and specificity of the assay were 97.8% and 100%, respectively. The OKNV assay is an accurate and rapid assay for identifying OXA-48-like, KPC, NDM, and VIM carbapenemases produced by Enterobacteriaceae isolates cultured on both SBA and the CHROMagar KPC media in the clinical microbiology laboratory.

Report this publication

Statistics

Seen <100 times