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A rapid factor VIII-related antigen electroimmunoassay. A micromethod using commercial antisera.

Authors
  • Moehring, C
Type
Published Article
Journal
American journal of clinical pathology
Publication Date
Nov 01, 1979
Volume
72
Issue
5
Pages
821–828
Identifiers
PMID: 315704
Source
Medline
License
Unknown

Abstract

A simple, rapid micromethod for determining Factor VIII-related antigen is outlined. The simplicity of the method, using commercial antisera, prepackaged buffer, plastic plates that readily accept the gel without precoating with agarose, and standard equipment makes the procedure adaptable to the routine laboratory. The results give a straight-line graph to the 0.50 units/ml range, and values as low as 0.03125 U/ml can be determined using log-log graph paper. A second range of standards gives values from 0.10 to 0.750 U/ml when plotted on semilog graph paper. A permanent record can be made after a first rapid visualization process, which permits reading the plates within 15 min of the two-and-a-half-hour electrophoresis time.

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