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Rapid enzymatic method for the measurement of mannitol in urine.

Authors
  • Blood, J
  • Ingle, A R
  • Allison, N
  • Davies, G R
  • Hill, P G
Type
Published Article
Journal
Annals of clinical biochemistry
Publication Date
Jul 01, 1991
Volume
28 ( Pt 4)
Pages
401–406
Identifiers
PMID: 1909850
Source
Medline
License
Unknown

Abstract

A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV less than 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0.994) between the two methods.

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