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Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device

Authors
  • Puri, Madhu1
  • Brar, Harsimran Kaur1
  • Mittal, Nimisha1
  • Madan, Evanka1
  • Srinivasan, Rajesh2
  • Rawat, Kapil2
  • Moulik, Srija3
  • Chatterjee, Mitali3
  • Gorthi, Sai Siva2
  • Muthuswami, Rohini1
  • Madhubala, Rentala1
  • 1 Jawaharlal Nehru University,
  • 2 Indian Institute of Science,
  • 3 Institute of Post-Graduate Medical Education and Research,
Type
Published Article
Journal
Journal of Biosciences
Publisher
Springer-Verlag
Publication Date
Sep 23, 2021
Volume
46
Issue
4
Identifiers
DOI: 10.1007/s12038-021-00211-0
PMCID: PMC8458559
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

L. donovani is an intracellular protozoan parasite, that causes visceral leishmaniasis (VL), and consequently, post-kala azar dermal leishmaniasis (PKDL). Diagnosis and treatment of leishmaniasis is crucial for decreasing its transmission. Various diagnostic techniques like microscopy, enzyme-linked immunosorbent assays (ELISA) and PCR-based methods are used to detect leishmaniasis infection. More recently, loop-mediated isothermal amplification (LAMP) assay has emerged as an ideal diagnostic measure for leishmaniasis, primarily due to its accuracy, speed and simplicity. However, point-of-care diagnosis is still not been tested with the LAMP assay. We have developed a portable LAMP device for the monitoring of Leishmania infection. The LAMP assay performed using our device can detect and amplify as little as 100 femtograms of L. donovani DNA. In a preliminary study, we have shown that the device can also amplify L. donovani DNA present in VL and PKDL patient samples with high sensitivity (100%), specificity (98%) and accuracy (99%), and can be used both for diagnostic and prognostic analysis. To our knowledge, this is the first report to describe the development and application of a portable LAMP device which has the potential to evolve as a point-of-care diagnostic and prognostic tool for Leishmania infections in future. Supplementary Information The online version contains supplementary material available at 10.1007/s12038-021-00211-0.

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