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Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR.

Authors
  • Kallonen, T
  • Mertsola, J
  • Mooi, F R
  • He, Q
Type
Published Article
Journal
Clinical Microbiology and Infection
Publisher
Elsevier
Publication Date
Oct 01, 2012
Volume
18
Issue
10
Identifiers
DOI: 10.1111/j.1469-0691.2012.04000.x
PMID: 22909320
Source
Medline
License
Unknown

Abstract

Bordetella pertussis strains with the pertussis toxin promoter allele ptxP3 have expanded and replaced resident ptxP1 strains in several European countries. We developed an allele-specific real-time PCR method to identify strains with the allele ptxP3, and investigated the emergence of ptxP3 strains by genotyping Finnish clinical isolates (n = 524) from 1953 to 2010. The first ptxP3 strain was detected in 1994, and has become predominant since 2003. Our results demonstrate that the allele-specific real-time PCR is a suitable method for rapid detection of ptxP3 strains, and show the emergence and establishment of ptxP3 strains in Finland.

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